Occurrence of Three Recombinant Strains of Potato Virus Y in Potato in Kazakhstan
نویسندگان
چکیده
منابع مشابه
Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187
Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
متن کاملDigoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
متن کاملSequence characteristics of potato virus Y recombinants.
Potato virus Y (PVY) is one of the most economically important plant pathogens. The PVY genome has a high degree of genetic variability and is also subject to recombination. New recombinants have been reported in many countries since the 1980s, but the origin of these recombinant strains and the physical and evolutionary mechanisms driving their emergence are not clear at the moment. The replic...
متن کاملErratum: The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus
[This corrects the article on p. 321 in vol. 32, PMID: 27493607.].
متن کاملDevelopment of RT-PCR based method for detection of potato virus Y in tobacco and potato
A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2020
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-03-19-0573-pdn